Aiming at the problem of fish migration in Lize Shipping Hub of Jialing River， this paper proposes the design scheme of vertical slot fishway which is more widely used,and uses three-dimensional water flow mathematical model to compare and analyze the hydraulic characteristics of four fishway layout types(changing the width of the pool chamber,the width of the vertical slit,and changing the dimensions or angle of the bulkhead and guide plate).The results show that under the conditions of the pool chamber width of 3.0 m,vertical seam width of 0.5 m,the partition length of 2.1 m,the guide plate length of 0.7 m,and upstream slope of 77° and 45° for the partition and the guide plate,the curvature radius of the main flow in the pool chamber is larger,the strength of the reflux zones on both sides is weaker,the flow velocity is more stable after passing through the rest room,and the water flow connection is smoother.The water flow conditions in the pool room and rest room meet the upstream requirements of fish.The research results can provide reference for similar fishway projects.