The lock chamber floor with filling culvert and energy dissipation facilities possesses the characteristics of 3D.According to method of the frame on elastic foundation or frame on uniform foundation in Lock hydraulic structure design specification,it is difficult to determine the stiffness of the frames and the 3D stress characteristics of floor is unable to simulate.This paper analyzes the force of ship’s lock chamber floor with filling culvert,and establishes the 3D finite element model by FEM analysis software,and calculates the stress and displacement of lock chamber floor.