Relying on a deep foundation pit project of the Qingyuan water control project second-line ship lock in the channel capacity expansion and upgrade project of North River，by carrying out real-time monitoring of the horizontal displacement，settlement，and vibration of existing ship lock and deep soil horizontal displacement between ship locks，this paper analyzes the disturbance characteristics of the second-line ship lock deep foundation pit excavation on adjacent first-line ship lock.The results show that in the process of second-line ship lock foundation pit excavation，the horizontal displacement and settlement of the lock chamber of the existing first-line ship lock follows the distribution law of large on both sides and small in the middle.When the excavation depth of the foundation pit is greater than 5 m，the settlement rate of the first-line ship lock increases significantly，and uneven settlement is likely to occur on both sides of the first-line ship lock.The soil horizontal displacement is closely related to the setting of the temporary axial force of the internal support，and the maximum cumulative deformation is 11.6 mm.The maximum vibration velocity of the first-line ship lock vibration monitoring point is 0.079 6 cms.During the second-line ship lock deep foundation pit excavation，the deformation disturbance on the adjacent first-line ship lock is controlled within a safe range，and the safety of the structure and operation of the ship lock is ensured.The research results can be used as a reference for the monitoring of ship locks.