To analyze the influence of daily regulation operation of Xiangjiaba Hydropower Station on the navigable flow conditions of downstream Zhongzui Wharf，a 1100 normal physical model is established to simulate the typical daily regulation operation of Xiangjiaba Hydropower Station，and the change process of navigable hydraulic factors such as velocity，water level and gradient of the water area of Zhongzui Wharf caused by daily regulation unsteady flow is analyzed.At the same time，combining the flow field distribution data and self-propelled ship model test，the influence of daily regulation on ship navigation and berthing in and out of the port is studied.The results show that when the power station conducts daily regulation according to the proposed water level variation，the water level variation of the wharf reach does not exceed the allowable value of the power station operation regulation，the flow conditions meet the requirements of ship navigation，and the ships can normally enter and leave the port for berthing operation，with little impact on the wharf operation.The research results can provide reference for daily regulation and operation of power stations.