Relying on the navigation project of Wujiang River，the hydraulics observation and ship test of the intermediate channel containing the long-distance navigation tunnel are carried out.Wave regulation of time and space of intermediate channel and ship navigation characteristics are revealed.The observation presents the intermediate channel wave propagation speed is about 5.0~6.8 ms.The wave amplitude is inversely proportional to the water width.The water surface at the two ends of the intermediate channel fluctuates the most，second in the navigation tunnel，and the large aqueduct intersection area fluctuates the least.The amplitude of the wave generated in the intermediate channel by the open and close of the chamber gate is within 2 cm，while that caused by ship navigation is basically within 10 cm.The main wave period is between 120 and 160 s.The water surface fluctuation has little impact on the underkeel clearance of the ship navigation.The normal navigation speed of the ship in the intermediate channel reaches the design index，and the maximum sinkage occurs in the continuous acceleration stage of the ship out of the chamber.The measured maximum sinkage of the upward and down is about 25 and 13 cm respectively.The maximum navigation sinkage of the ship in the intermediate channel is basically within 10 cm.The underkeel clearance is rich and the design of the navigable tunnel section is generally reasonable.