The study of the three-dimensional cylinder flow mainly focuses on the degree of the simulation accuracy，and the analyses of the single-cylinder wake vortex structure. However，there are less research on characteristics of the three-dimensional three tandem cylinders flow. The simulation on three tandem cylinders turbulence model from three dimensions is carried out at the different spacing ratios by taking Reynold number as 2.25×106 by FLUENT software. Gambit software is used to model the flow field，and the grid of the water near the cylinder is densified，and a three-dimensional LES simulation is carried out for three tandem cylinders in series. Three different L/D（L is the spacing between the cylinders and D is the diameter of the cylinder） are selected as 1.0，2.0 and 3.0，and three different heights of tangent planes （0 m，2.25 m and 4.40 m from the water surface） are selected in the 3D simulation to study the turbulence of the velocity field，pressure field and vorticity field characteristics. The results show that the three-dimensional simulation confirms the significant changing of vorticity of the surface，and the backflow area behind the continuous cylinder is strongly correlated with the spacing ratio. At the same time，the velocity fields of the three different tangent planes have small changes，and the pressure field changes little.