In recent years，the contradiction between bridge site selection and the requirements for the axial spacing between adjacent over-river structures in Clause 5.1.2 of the Navigation Standard of Inland Waterway has become increasingly prominent. In response to this problem，the study takes the Yicheng Hanjiang River Bridge of the Xiangyang-Jingmen high-speed railway as an example to analyze the construction conditions such as the change in the river channel，waterway maintenance，flow conditions，and density of water transport in the river section of the bridge. On this basis，a simulation experiment of ship navigation is carried out. The results reveal that on the premise of ensuring the safety of ship navigation and bridges，we can appropriately adjust the requirements for the spacing between adjacent bridges according to the construction conditions in the river section of the bridge and the layout of the navigable span. This paper demonstrates the limitations of the requirements for the axial spacing between adjacent over-river structures in the Navigation Standard of Inland Waterway and puts forward suggestions for its revision.