The destuffing of container twistlocks is an essential step during the process of container ship loading and unloading at specialized container terminals. The stationary and concentrated container twistlock destuffing technology，commonly used in the automated container handling system adopting the automatic quayside container crane with a single trolley，has a few disadvantages，such as complex traffic organization and low safety and turnover efficiency of traffic flow. On the basis of the Nansha automated container terminal in Guangzhou Port，we put forward a new plan of mobile and concentrated container twistlock destuffing technology and elaborately describe the plan design in terms of the layout and operation process. The new plan uses intelligent equipment scheduling and electronic locking technology to realize the isolation and diversion of people and automated vehicles during container twistlock destuffing，which boasts simple traffic organization and high turnover efficiency of traffic flow. It can provide a reference for the design of container twistlock destuffing technology for similar automated container terminals.