Analyzing navigation-obstructing characteristics is particularly critical for the design of a sharp bend channel in a water-level-fluctuating zone upstream from a hydro-junction. Considering the complicated water flow conditions of the Matouling sharp bend upstream from the Wuxi Hydro-junction，we adopt the technical means of combining a two-dimensional planar mathematical water flow model with a ship model to study the navigation-obstructing characteristics of the bend river under different water level combinations. An optimization scheme is proposed by analyzing the channel depth and flow rate. The results show that the navigable design water level of a stage-fluctuating zone upstream from a hydro-junction is greatly affected by the operation scheduling plan of the hydro-junction. Adjusting the route on a sharp bend channel is an effective idea for optimizing navigable conditions. This research serves as a reference for the construction of similar projects.