By establishing a three-dimensional turbulence mathematical model，the hydraulic characteristics of the fishway pool of Bengbu sluice with the baffle layout are calculated to study the flow pattern，velocity of the pool and the resting pool，and the maximum vertical slot velocity. In particular，the velocities of 920 measuring points in 20 cross sections of the upper，middle and lower water layers of the typical pool and resting pool are statistically analyzed，and the transverse，longitudinal and vertical velocities of 140 measuring points are finely analyzed. The influence of the slope of the pool on the hydraulic characteristics of the pool is also studied. The results show that：1）The velocity and flow pattern of the upper，middle and lower water layers in fishway pool and resting pool are basically the same. The lower the water layer is，the higher the main flow velocity is. 2）The vertical velocity of flow in the pool is very small，and the flow presents two-dimensional characteristics. 3）The main flow velocity of the resting pool is equivalent to that of the main flow velocity of the pool （only 3.5% lower on average），but the area of the low-velocity zone is significantly increased （54% higher）.4）The slope of the pool slowed from 1:100 to 1:140，and the maximum velocity of vertical joint decreased by 16.9%. 5）The slope of the fishway pool of Bengbu Sluice is recommended to be 1:100~1:120 to meet the demand of fish upward tracking.