Regarding the relocation and site selection of Karachi Fishing Port，this paper adopts a multi-factor comprehensive evaluation method to analyze and evaluate four alternative port sites for port site recommendation. In view of the characteristics of fishing ports，we should consider the convenience of transportation between a fishing port and the concentrated residential area of fishery workers as well as the impact of the fishing port on the surrounding environment when analyzing the location of the fishing port. Moreover，the relationship with the planned land use and the environmental impact on the planned area must be involved for the consideration of coordination between site selection and planning of a fishing port. Finally，this paper provides methods for the quantification of all factors involved in site selection for reference.