In order to analyze the influence of container slot assignment strategy of container terminal with automated yard on terminal operation，we apply the computer simulation method combined with the actual projects to study. We summarize the common slot assignment strategies from related research and operating project，establish the system simulation models of container terminals in Peru and Greece based on Flexterm simulation platform，simulate the processes of automated yard and ship-to-shore operating under different slot assignment strategies，and collect the ratios of efficiency and states of main equipment after simulation tests. The results show that there are no specific patterns for the influence of slot assignment strategy on terminal operation，and the slot assignment strategy should be carefully chose according to the actual condition of research program and research target.