Given how to scientifically evaluate the operational supporting capacity of the locks，we study the evaluation system of the operational supporting of the locks.Focusing on the evaluation of the operational supporting capacity of the lock，starting from the core factors such as management mechanism，equipment management，navigation supporting and navigation service，we use the methods of hierarchical analysis，Delphi method，etc.，adopt the research means combining qualitative analysis with quantitative calculation，comprehensively determine the evaluation index and weight，establish the comprehensive evaluation method of the operational supporting capacity of the locks，and apply the method to a large single-stage lock in China to derive the comprehensive evaluation of the operational supporting capacity of the lock. The results show that the comprehensive evaluation of the operational supporting capacity of a large single-stage ship lock is excellent. The evaluation method basically conforms to the practical experience of ship lock field and management unit in recent years，and can be applied to similar projects.