The operating conditions of filling and emptying valve in high head navigation lock are very complex during operation due to hydrodynamic load，which directly affects the hoist load of the valve. As the hydrodynamic hoist load is the key design parameter to determine the capacity and dead load of the valve，it is necessary to carry out special research. 1）Specific to reverse tainter valve，the influences of valve opening and closing rate，culvert type at valve section，and the water head on the hydrodynamic hoist load are analyzed. 2）By changing structure parameters such as panel structure，the shape of valve’s bottom edge，and arms wrapping，the influence of the shape resistance of valve on hydrodynamic hoist load are studied，and the relevant laws are obtained. 3）The field observation data of the valve hydrodynamic hoist load of the Three Gorges ship lock is used to verify the model test values. The results show that the two results are in good agreement，and the capacity and a dead load of filling and emptying valve in the Three Gorges ship lock can meet the requirements of the hydrodynamic operation.