The submerged dike，spur dike and bottom protection zone are applied in the Luochengzhou waterway regulation project on the 12.5 m deepwater channel of the Yangtze River below Nanjing to prevent the right branch from further scouring and development，enhance the power of shallow section of left branch and improve the channel conditions. In order to demonstrate the regulation effect of the implementation of Luochengzhou waterway regulation project，we compare the shoal and channel evolution，the flow and sediment diversion ratios of branched channels，the shoal consolidation effect and the improvement of channel navigation conditions before and after the implementation of the Luochengzhou waterway regulation project based on the measured topographic and hydrological data. The results show that after the implementation of the project，the sediment deposited in the head of sandbar and the shelter area of the spur dike，and the trend of erosion and retreat of the head of sandbar are effectively curbed. The diversion ratio of the left branch increases by about 4%，the velocity of flow in the spur dike’s pick-up area increases by about 0.2 m/s，and the water depth increases from 10.5 m to 12.5 m. The regulation aims are achieved in general through the regulation buildings，but attention should be paid to the impact of the deposition at Sanyiqiao marginal bank on the deepwater channel.