In order to verify whether Daoyunhai waterway regulation project satisfies the navigation requirements of the regulation standards,we select the Doulang-Chuancha Bridge section as the experimental section,apply the 500 tons full load ship designed for this section as the trial ship to carry out the actual seaworthiness test.We measure the depth and topography,surface velocity and direction,navigation mark distribution and water level of the experimental section.We also measure the navigation indexes and navigation status of the test ship in real time,such as the navigation track,the speed of the opposite bank,the rudder angle,the drift angle,the safe distance between the ship and the bank when the test ship navigates up and down the designed route.According to the navigation rules and standards,we analyze and evaluate the rationality of the technical parameters and the distribution of navigation marks after the regulation.The results show that the regulation effect of this test section satisfies the navigation requirements of the regulation standard.The achievement can provide references for the similar projects.