Concerning the problem of competition and cooperation relationships of ports，traditional research mainly uses theoretical model architecture or the game strategy and fails in the in-depth analysis of the situation of competition and cooperation relationship of ports.Ecological niche theory effectively studies species growth，relationship between species and community development in the ecological system.According to the ecological niche theory，we analyze the characteristics of port niche and the law of development and build the factor dimension index system of the port ecological niche to carry out the bionic research of competition and cooperation relationship of ports.Based on the theory of niche trend，niche breadth and niche overlap，we calculate the port niche.Using the competitive strategy of niche expansion，co-evolution and dislocation separation，we provide reference for improving the competition and cooperation relationship of ports and achieve collaborative symbiosis between ports.