At present, more and more bridges are built on the Yangtze River, thus the building density is large in main urban areas and developed regions, which leads to the phenomenon that the spacing between the new bridge and old riverside buildings such as port is insufficient. On the basis of GB 50139—2004 Navigation standard of inland waterway, we analyze the method of the research about spacing between bridge and riverside buildings such as port, anchorage ground, slipway and water-intake. Furthermore, the requirement about the bridge number control and the navigation opening arrangement are discussed to reduce the impact on navigation and better serve the inland navigation industry and economic construction along the Yangtze River.