In order to improve the berth throughput capacity of the ore wharf in Dongjiakou Port area of Qingdao Port and enhance the efficiency of unloading operations, this paper considers the high risk factor for two 400, 000-ton ore ships to conduct mooring operations in a port with a limited basin under the background that the utilization rate of the ore berth in Dongjiakou Port area is currently high, and no new wharf is built. Based on the ship maneuvering simulation test, the paper simulates a mooring operation test of the 400, 000-ton ore ships under different conditions of wind, wave, and current and determines the key elements of 400, 000-ton ore ship mooring operations according to the test results. The results show that under determined key elements of mooring operations, Dongjiakou Port area can safely carry out the 400, 000-ton ore ship mooring operations.