Sheyimen Channel is located in the northern sea area of Ningbo-Zhoushan Port.The channel uses the world’s largest ore ship as the design ship type,and it is the first 400，000 DWT channel designed and put into operation in China.Restricted by the islands,reefs and port layout,the channel flow condition is complicated,and navigation and berthing conditions are bad.Because the waterway condition is one of the most complicated navigable waters in Zhoushan,the design of the channel is very difficult.In this paper,we make a comprehensive analysis of the characteristics and difficulties of the channel,reasonable ship traffic organization and navigation mode are put forward,we optimize the channel design scheme by ship simulation tests,determine the window period of navigation by theoretical calculation and real ship testing,and obtain a real-time flow observation scheme during the test run.