Due to the scale effect between the model and prototype of ship lock,the huge dimension and the new type energy dissipation measures of the Guiping second lane lock,for knowing the actual hydraulic characteristics and guaranteeing the safety and efficiency of the lock,by prototype observation and full scale ship test,we observe and analyze the hydraulic characteristics,ship berthing conditions and also the interaction effect between the first and second lane lock.The results indicate that:under the current operation mode,each hydraulic characteristics of the second lane lock can basically satisfy the demands of the standards and design,but there will be some influences on the gate of the first lane lock when the second lane lock is emptying.More concerns shall be given to the influence of the overshooting on the ship’s berthing safety.