The plan layout and navigable condition in the water diversion project from the Yangtze River to the Hanjiang River are analyzed by a combination of the tidal current physical model test, remote-control selfpropulsive ship model, mathematical model and maneuvering mathematical model. This paper expounds the navigation condition of the junction of the Yangtze River and the water diversion project, downstream channel of Longzhouyuan lock and navigable spans of Jingjiang dyke, analyzes the influential factors of the navigable flow condition in the design, and proposes the optimal layout scheme which satisfies the requirement of safe navigation through taking a variety of engineering measures to improve the navigable floe condition to counter the disadvantages of the design scheme.